Dental Hypotheses

ORIGINAL HYPOTHESIS
Year
: 2016  |  Volume : 7  |  Issue : 1  |  Page : 12--14

Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis


Jafar Kolahi 
 Independent Research Scientist, Founder and Managing Editor of Dental Hypotheses, Isfahan, Iran

Correspondence Address:
Jafar Kolahi
No. 24, Faree 15, Pardis, Shahin Shahr, Isfahan - 83179 18981
Iran

Introduction: Dental caries (tooth decay) remains one of the most common chronic infectious disease in the world. Disclosure of camouflaged cariogenic bacteria will be a great motivation for better oral hygiene. The Hypothesis: At present, lux transposon cassette, Tn4001 luxABCDE Kmr, is available that could be used for stable bioluminescent transformation of a wide range of gram-positive bacteria, e.g. Streptococcus mutans and Lactobacillus. After this step, sensitive charge-coupled device (CCD) camera could be used to detect the low levels of light emitted from bioluminescent cariogenic bacteria. Living imaging software would be used for analysis and three-dimensional (3D) reconstruction of images. Evaluation of the Hypothesis: Entrance of transgenic organisms into the oral cavity should be done with great caution. Ethical consideration is necessary and primary animal studies are required. The main limitation of this technique will be oxygen. As mentioned previously, bioluminescent reactions need oxygen. Hence, bioluminescent imaging cannot be used for anaerobic bacteria, e.g., Streptococcus sobrinus.


How to cite this article:
Kolahi J. Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis.Dent Hypotheses 2016;7:12-14


How to cite this URL:
Kolahi J. Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis. Dent Hypotheses [serial online] 2016 [cited 2020 Aug 8 ];7:12-14
Available from: http://www.dentalhypotheses.com/article.asp?issn=2155-8213;year=2016;volume=7;issue=1;spage=12;epage=14;aulast=Kolahi;type=0