Dental Hypotheses

ORIGINAL RESEARCH
Year
: 2019  |  Volume : 10  |  Issue : 4  |  Page : 103--107

Molecular Detection of the Predatory Bacterium Bdellovibrio bacteriovorus from Dental Biofilms


Claudio Passariello1, Serena Schippa1, Dario Di Nardo2, Antonella Polimeni2, Luca Testarelli2 
1 Department of Public Health and Infectious Diseases, ‘Sapienza’ University of Rome, Rome, Italy
2 Department of Oral and Maxillo-Facial Sciences, ‘Sapienza’ University of Rome, Via Caserta, Rome, Italy

Correspondence Address:
DDS, PhD Dario Di Nardo
Department of Oral and Maxillo-Facial Sciences, Sapienza University of Rome, Via Caserta, 6 – 00161 Rome
Italy

Introduction: The oral microbiome is a complex community whose composition results from multiple interactions among different microorganisms and with the host. Predatory prokaryotes are recognized as important balancing factors in different ecosystems. Among them Bdellovibrio bacteriovorus received special attention for its strong predatory behaviour against many human pathogens in the absence of any toxic or pathogenic effect, so that it was proposed as a live probiotic/antibiotic agent. This work aimed to evaluate if B. bacteriovorus is detected from samples of human oral and dental biofilm. Materials and Methods: Samples of oral and dental biofilm were obtained from 20 adults of both sexes and processed for extraction of metagenomic DNA, to be used as templates for B. bacteriovorus-specific PCR reactions. Specificity of amplification products was confirmed by sequencing. Results: All 20 dental biofilm samples and 12 of 20 (60%) oral biofilm samples were resulted PCR positive. The sequences of 17 of 32 PCR products (53.1%) showed 100% identity with the reference sequence; the sequences of 11 of 32 PCR products (34.4%) showed ≥99% identity, while the remaining 4 products (12.5%) showed identities ranging between <99% and ≥97%. Conclusions: This is the first survey specifically reporting the presence of B. bacteriovorus in the human oral cavity and suggests that bacterial predation is a relevant balancing factor for the oral microbiota. Demonstration that B. bacteriovorus is able to colonize the oral cavity gives strength to proposals of its use as a probiotic/antibiotic in the prevention/treatment of selected oral diseases.


How to cite this article:
Passariello C, Schippa S, Di Nardo D, Polimeni A, Testarelli L. Molecular Detection of the Predatory Bacterium Bdellovibrio bacteriovorus from Dental Biofilms.Dent Hypotheses 2019;10:103-107


How to cite this URL:
Passariello C, Schippa S, Di Nardo D, Polimeni A, Testarelli L. Molecular Detection of the Predatory Bacterium Bdellovibrio bacteriovorus from Dental Biofilms. Dent Hypotheses [serial online] 2019 [cited 2020 Feb 27 ];10:103-107
Available from: http://www.dentalhypotheses.com/article.asp?issn=2155-8213;year=2019;volume=10;issue=4;spage=103;epage=107;aulast=Passariello;type=0